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Funzionale e morfologica Valutazione della membrana innervazione da Phrenic Motor Neurons
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Neuroscience
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JoVE Journal Neuroscience
Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons
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Funzionale e morfologica Valutazione della membrana innervazione da Phrenic Motor Neurons

italiano

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17,020 Views

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09:43 min

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May 25, 2015

DOI:

10.3791/52605-v

DOI:
10.3791/52605-v

•

09:43 min
May 25, 2015

•

20 Views
, , ,
1Department of Neuroscience, Farber Institute for Neurosciences,Sidney Kimmel Medical College at Thomas Jefferson University, 2Department of Biology,Arcadia University

Summary

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Compound muscle action potential recording quantitatively assesses functional diaphragm innervation by phrenic motor neurons. Whole-mount diaphragm immunohistochemistry assesses morphological innervation at individual neuromuscular junctions. The goal of this protocol is to demonstrate how these two powerful methodologies can be used in various rodent models of spinal cord disease.

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Diaphragm InnervationPhrenic Motor NeuronsCompound Muscle Action PotentialNeuromuscular JunctionImmunohistochemistryDenervationReinnervationRodent ModelsCNS DiseasePNS Disease

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Cite this Article

Martin, M., Li, K., Wright, M. C., Lepore, A. C. Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons. J. Vis. Exp. (99), e52605, doi:10.3791/52605 (2015).

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