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Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons
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Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

17,042 Views

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09:43 min

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May 25, 2015

DOI:

10.3791/52605-v

DOI:
10.3791/52605-v

•

09:43 min
May 25, 2015

•

20 Views
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1Department of Neuroscience, Farber Institute for Neurosciences,Sidney Kimmel Medical College at Thomas Jefferson University, 2Biyoloji Bölümü,Arcadia University

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Compound muscle action potential recording quantitatively assesses functional diaphragm innervation by phrenic motor neurons. Whole-mount diaphragm immunohistochemistry assesses morphological innervation at individual neuromuscular junctions. The goal of this protocol is to demonstrate how these two powerful methodologies can be used in various rodent models of spinal cord disease.

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Diaphragm InnervationPhrenic Motor NeuronsCompound Muscle Action PotentialNeuromuscular JunctionImmunohistochemistryDenervationReinnervationRodent ModelsCNS DiseasePNS Disease

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Martin, M., Li, K., Wright, M. C., Lepore, A. C. Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons. J. Vis. Exp. (99), e52605, doi:10.3791/52605 (2015).

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