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Trasplante de células musculares normales y malignas en inmunológico comprometido adultos de pez cebra
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish
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Trasplante de células musculares normales y malignas en inmunológico comprometido adultos de pez cebra

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10,733 Views

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09:39 min

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December 26, 2014

DOI:

10.3791/52597-v

DOI:
10.3791/52597-v

•

09:39 min
December 26, 2014

•

3 Views
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1Molecular Pathology, Cancer Center and Center for Regenerative Medicine,Massachusetts General Hospital, 2Harvard Stem Cell Institute, 3GABBA – Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto

Summary

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Here, we present a protocol for cell transplantation of zebrafish skeletal muscle and embryonal rhabdomyosarcoma (ERMS) into adult immune compromised rag2E450fs homozygous mutant zebrafish. This protocol allows for the efficient analysis of regeneration and malignant transformation of muscle cells.

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ZebrafishCell TransplantationSkeletal MuscleRhabdomyosarcomaRag2 MutantImmune CompromisedEngraftmentRegenerationCancer

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Tenente, I. M., Tang, Q., Moore, J. C., Langenau, D. M. Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish. J. Vis. Exp. (94), e52597, doi:10.3791/52597 (2014).

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