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Atacante Genetics telas usando macrófagos para Identificar<em> Toxoplasma gondii</em> genes importantes celular para Resistance to-IFN-γ Dependente Immunity Autónoma
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Immunology and Infection
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JoVE Journal Immunology and Infection
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity

Atacante Genetics telas usando macrófagos para Identificar<em> Toxoplasma gondii</em> genes importantes celular para Resistance to-IFN-γ Dependente Immunity Autónoma

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10,923 Views

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11:21 min

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March 12, 2015

DOI:

10.3791/52556-v

DOI:
10.3791/52556-v

•

11:21 min
March 12, 2015

•

2 Views
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1Department of Microbiology and Immunology,New York Medical College

Summary

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Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.

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Walwyn, O., Skariah, S., Lynch, B., Kim, N., Ueda, Y., Vohora, N., Choe, J., Mordue, D. G. Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity. J. Vis. Exp. (97), e52556, doi:10.3791/52556 (2015).

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