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正向遗传学屏风使用巨噬细胞识别<em>弓形虫</em>基因的重要的抗IFN-γ依赖性细胞免疫自主
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Immunology and Infection
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JoVE Journal Immunology and Infection
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
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正向遗传学屏风使用巨噬细胞识别<em>弓形虫</em>基因的重要的抗IFN-γ依赖性细胞免疫自主

English

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10,923 Views

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11:21 min

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March 12, 2015

DOI:

10.3791/52556-v

DOI:
10.3791/52556-v

•

11:21 min
March 12, 2015

•

2 Views
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1Department of Microbiology and Immunology,New York Medical College

Summary

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Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.

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Walwyn, O., Skariah, S., Lynch, B., Kim, N., Ueda, Y., Vohora, N., Choe, J., Mordue, D. G. Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity. J. Vis. Exp. (97), e52556, doi:10.3791/52556 (2015).

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