Journal
/
Developmental Biology
/
造血細胞の自動定量化 - 脱灰骨の組織学的画像における間質細胞の相互作用
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
Please note that all translations are automatically generated. Click here for the English version.

造血細胞の自動定量化 - 脱灰骨の組織学的画像における間質細胞の相互作用

日本語

Automatically Generated

11,385 Views

•

09:31 min

•

April 08, 2015

DOI:

10.3791/52544-v

DOI:
10.3791/52544-v

•

09:31 min
April 08, 2015

•

1 Views
, , , ,
1Immunodynamics,German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics,German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging,Charité – University of Medicine

Summary

Automatically generated

A strategy to quantitatively analyze histological data in the bone marrow is presented. Confocal microscopy of fluorescently labeled cells in tissue sections results in 2-dimensional images, which are automatically analyzed. Co-localization analyses of different cell types are compared to data from simulated images, giving quantitative information about cellular interactions.

Explore More Videos

Automated QuantificationCell-cell InteractionsHematopoietic CellsStromal CellsBone MarrowConfocal MicroscopyHistological AnalysisCell RecognitionNeighborhood AnalysisCo-localizationRandom Positioning

Read Article

Cite this Article

Zehentmeier, S., Cseresnyes, Z., Escribano Navarro, J., Niesner, R. A., Hauser, A. E. Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone. J. Vis. Exp. (98), e52544, doi:10.3791/52544 (2015).

Download .ris file

Copy

Share Video

.

Copy