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JoVE Journal Developmental Biology
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
 

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Article DOI: 10.3791/52544-v • 09:31 min • April 8th, 2015
April 8th, 2015 •
1, 2,3, 4, 2, 1,5
1Immunodynamics, German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics, German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine

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Resumo
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A strategy to quantitatively analyze histological data in the bone marrow is presented. Confocal microscopy of fluorescently labeled cells in tissue sections results in 2-dimensional images, which are automatically analyzed. Co-localization analyses of different cell types are compared to data from simulated images, giving quantitative information about cellular interactions.

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Automated Quantification Cell-cell Interactions Hematopoietic Cells Stromal Cells Bone Marrow Confocal Microscopy Histological Analysis Cell Recognition Neighborhood Analysis Co-localization Random Positioning
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