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Manipulação e<em> In Vitro</em> Maturação<em> Xenopus laevis</em> Oócitos, seguido de injeção intracitoplasmática de espermatozóide, para estudar o desenvolvimento embrionário
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Manipulação e<em> In Vitro</em> Maturação<em> Xenopus laevis</em> Oócitos, seguido de injeção intracitoplasmática de espermatozóide, para estudar o desenvolvimento embrionário

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09:22 min

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February 09, 2015

DOI:

10.3791/52496-v

DOI:
10.3791/52496-v

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09:22 min
February 09, 2015

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1Wellcome Trust/Cancer Research UK Gurdon Institute,University of Cambridge, 2Department of Zoology,University of Cambridge

Summary

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We describe methods of manipulating Xenopus laevis immature oocytes, in vitro maturation of oocytes to eggs, and intracytoplasmic sperm injection. This protocol allows degradation of some maternal proteins and overexpression of genes of interest at fertilization, and hence is valuable to study roles of specific factors in early embryonic development.

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Miyamoto, K., Simpson, D., Gurdon, J. B. Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development. J. Vis. Exp. (96), e52496, doi:10.3791/52496 (2015).

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