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Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

DOI:
10.3791/52496-v

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09:22 min

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February 09, 2015

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, ,
1Wellcome Trust/Cancer Research UK Gurdon Institute,University of Cambridge, 2Department of Zoology,University of Cambridge

Capítulos

  • 00:05Título
  • 01:14Preparations
  • 03:47Injecting Oocytes with Oligos and Oocyte Maturation
  • 05:55Intracytoplasmic Sperm Injection
  • 08:12Results: ICS Embryo Survival
  • 09:01Conclusion

Summary

Traducción Automática

Traducción Automática

We describe methods of manipulating Xenopus laevis immature oocytes, in vitro maturation of oocytes to eggs, and intracytoplasmic sperm injection. This protocol allows degradation of some maternal proteins and overexpression of genes of interest at fertilization, and hence is valuable to study roles of specific factors in early embryonic development.

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Xenopus LaevisOocytesIn Vitro MaturationIntracytoplasmic Sperm InjectionICSIEmbryonic DevelopmentMaternal FactorsGene InterferenceAntisense OligonucleotidesMRNA InjectionProtein ExpressionSwimming Tadpole

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