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Generazione di Murine Cardiac pacemaker aggregati di cellule Sulla base ES-Cell-programmazione in combinazione con MYH6-Promoter-Selection
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection
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Generazione di Murine Cardiac pacemaker aggregati di cellule Sulla base ES-Cell-programmazione in combinazione con MYH6-Promoter-Selection

italiano

Automatically Generated

9,562 Views

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08:52 min

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February 17, 2015

DOI:

10.3791/52465-v

DOI:
10.3791/52465-v

•

08:52 min
February 17, 2015

•

7 Views
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1Reference and Translation Center for Cardiac Stem Cell Therapy,University of Rostock

Summary

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Questo protocollo descrive come produrre funzionale del seno tessuto nodale da cellule murine staminali pluripotenti (PSC). T-box3 (TBX3) sovraespressione più cardiaca miosina pesante catena (MYH6) selezione antibiotica promoter porta a aggregati altamente puri di cellule pacemaker. Questi "indotte-seno-atriale-corpi" ("iSABs") contengono più di 80% di cellule pacemaker, mostrano fortemente aumentato i tassi di battere e sono in grado di camminare miocardio ex vivo.

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Murine Cardiac Pacemaker CellsES-cell-programmingMyh6-promoter-selectionInduced-sinoatrial-bodies (iSABs)Sinus Node Inducer TBX3Pacemaker CellsSpontaneous ContractionNodal Cells

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Cite this Article

Rimmbach, C., Jung, J. J., David, R. Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection. J. Vis. Exp. (96), e52465, doi:10.3791/52465 (2015).

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