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Journal / Developmental Biology / Generation of Murine Cardiac Pacemaker Cell…
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Developmental Biology

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JoVE Journal Developmental Biology
Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection
 

Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection

Article DOI: 10.3791/52465-v • 08:52 min • February 17th, 2015
February 17th, 2015 •
1, 1, 1
1Reference and Translation Center for Cardiac Stem Cell Therapy, University of Rostock

Kapitel

Summary
Automatic Translation

This protocol describes how to produce functional sinus nodal tissue from murine pluripotent stem cells (PSC). T-Box3 (TBX3) overexpression plus cardiac Myosin-heavy-chain (Myh6) promoter antibiotic selection leads to highly pure pacemaker cell aggregates. These “Induced-sinoatrial-bodies” (“iSABs”) contain over 80% pacemaker cells, show highly increased beating rates and are able to pace myocardium ex vivo.

Tags

Murine Cardiac Pacemaker Cell Aggregates ES-cell Programming Myh6-promoter-selection Sick Sinus Syndrome Artificial Pacemakers Biological Pacemakers PSCs Embryoid Bodies (EBs) Forward Programming Sinus Node Inducer TBX3 Antibiotic Selection Induced-sinoatrial-bodies (iSABs) Physiologically Functional Pacemaker Cells Nodal Cells Murine Myocardium Ex Vivo In Vitro Drug Testing Heart Tissue Engineering
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