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Une approche pour l'évaluation optogénétiques formation des connexions neuronales dans un système de co-culture
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System
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Une approche pour l'évaluation optogénétiques formation des connexions neuronales dans un système de co-culture

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13,087 Views

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11:22 min

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February 17, 2015

DOI:

10.3791/52408-v

DOI:
10.3791/52408-v

•

11:22 min
February 17, 2015

•

8 Views
, , , , ,
1Neuroscience & Behavioral Disorders,Duke-NUS Graduate Medical School, 2Lee Kong Chian School of Medicine,Nanyang Technological University

Summary

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A protocol to generate a co-culture system consisting of neurons derived from induced pluripotent stem cells (iPSCs), primary cortical neurons and astrocytes is described. This co-culture system allows detection of the formation of synaptic contacts and circuits between new, iPSC-derived neurons and pre-existing cortical neurons expressing channelrhodopsin-2.

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OptogeneticsNeuronal ConnectionsCo-culture SystemInduced Pluripotent Stem Cells (iPSCs)Neural Progenitor Cells (NPCs)AstrocytesCortical NeuronsChannelrhodopsin-2 (ChR2)Tandem Dimer Tomato (tdTomato)Synaptic CurrentsPhotostimulation

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Su, C. T. E., Yoon, S., Marcy, G., Chin, E. W. M., Augustine, G. J., Goh, E. L. K. An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System. J. Vis. Exp. (96), e52408, doi:10.3791/52408 (2015).

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