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Une approche pour l'évaluation optogénétiques formation des connexions neuronales dans un système de co-culture
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

Une approche pour l'évaluation optogénétiques formation des connexions neuronales dans un système de co-culture

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11:22 min

February 17, 2015

DOI:

11:22 min
February 17, 2015

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Summary

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A protocol to generate a co-culture system consisting of neurons derived from induced pluripotent stem cells (iPSCs), primary cortical neurons and astrocytes is described. This co-culture system allows detection of the formation of synaptic contacts and circuits between new, iPSC-derived neurons and pre-existing cortical neurons expressing channelrhodopsin-2.

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