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An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System
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An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System
Kapitel
- 00:05Titel
- 02:12Cortical Tissue Dissociation
- 03:52Electroporation and Plating of Primary Cortical Neurons
- 05:14Differentiation of Human Neural Progenitor Cells into Neurons in Co-culture
- 06:55Electrophysiological Recordings of Induced Pluripotent Stem Cell-derived Neurons
- 09:05Results: Co-culture System to Assess Neuronal Connection Formation
- 10:53Conclusion
A protocol to generate a co-culture system consisting of neurons derived from induced pluripotent stem cells (iPSCs), primary cortical neurons and astrocytes is described. This co-culture system allows detection of the formation of synaptic contacts and circuits between new, iPSC-derived neurons and pre-existing cortical neurons expressing channelrhodopsin-2.
