Journal
/
Immunology and Infection
/
침투 림프구의 분석을위한 비 효소 해리 신선한 인간의 조직에 대한 간단하고 빠른 프로토콜
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
Please note that all translations are automatically generated. Click here for the English version.

침투 림프구의 분석을위한 비 효소 해리 신선한 인간의 조직에 대한 간단하고 빠른 프로토콜

English

Automatically Generated

19,772 Views

•

07:29 min

•

December 06, 2014

DOI:

10.3791/52392-v

DOI:
10.3791/52392-v

•

07:29 min
December 06, 2014

•

4 Views
, , , , , , , , , ,
1Molecular Immunology Unit,Université Libre de Bruxelles, 2Institut Jules Bordet,Université Libre de Bruxelles, 3Institut d’Immunologie Médicale,Université Libre de Bruxelles, 4Flow Cytometry Core Facility,Université Libre de Bruxelles

Summary

Automatically generated

This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.

Explore More Videos

Lymphocyte IsolationTumor Infiltrating LymphocytesBreast CancerImmune ResponseFlow CytometryProtein AnalysisCytokinesChemokines

Read Article

Cite this Article

Garaud, S., Gu-Trantien, C., Lodewyckx, J., Boisson, A., De Silva, P., Buisseret, L., Migliori, E., Libin, M., Naveaux, C., Duvillier, H., Willard-Gallo, K. A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes. J. Vis. Exp. (94), e52392, doi:10.3791/52392 (2014).

Download .ris file

Copy

Share Video

.

Copy