Dergi
/
İmmünoloji ve Enfeksiyon
/
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes

19,782 Views

•

07:29 min

•

December 06, 2014

DOI:

10.3791/52392-v

DOI:
10.3791/52392-v

•

07:29 min
December 06, 2014

•

4 Views
, , , , , , , , , ,
1Molecular Immunology Unit,Université Libre de Bruxelles, 2Institut Jules Bordet,Université Libre de Bruxelles, 3Institut d’Immunologie Médicale,Université Libre de Bruxelles, 4Flow Cytometry Core Facility,Université Libre de Bruxelles

Özet

Automatically generated

This protocol describes the rapid non-enzymatic dissociation of fresh human tissue fragments for qualitative and quantitative assessment of CD45+ cells (lymphocytes/leukocytes) present in various normal and malignant human tissues. Additionally, the supernatant obtained from the primary tissue homogenate can be collected and stored for further analysis or experimentation.

Explore More Videos

Lymphocyte IsolationTumor Infiltrating LymphocytesBreast CancerImmune ResponseFlow CytometryProtein AnalysisCytokinesChemokines

Read Article

Cite this Article

Garaud, S., Gu-Trantien, C., Lodewyckx, J., Boisson, A., De Silva, P., Buisseret, L., Migliori, E., Libin, M., Naveaux, C., Duvillier, H., Willard-Gallo, K. A Simple and Rapid Protocol to Non-enzymatically Dissociate Fresh Human Tissues for the Analysis of Infiltrating Lymphocytes. J. Vis. Exp. (94), e52392, doi:10.3791/52392 (2014).

Download .ris file

Copy

Share Video

.

Copy