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Prøvepreparering Strategier for massespektrometri Imaging av 3D-Cell Kultur Modeller
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
DOI:

08:14 min

December 05, 2014

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Chapters

  • 00:05Title
  • 01:28Three-dimensional Cell Culture and Preparation of Agarose Coated 96 Well Plates
  • 02:32Seed and Harvest the Three-dimensional Cultures and Embed in Gelatin
  • 04:11Slice and Thaw Mount the Gelatin-embedded Three-dimensional Cell Cultures for Mass Spectrometric Imaging
  • 05:03Matrix Application and Sample Preparation for MALDI Imaging
  • 05:42MALDI-MSI Analysis Using a MALDI-TOF System and Optional Data Analysis Methods
  • 07:28Results: Mass Spectrometry Imaging of Three-dimensional Cell Cultures
  • 07:49Conclusion

Summary

Automatic Translation

Udødeligkreftcellelinjer kan dyrkes som 3D cellekulturer, en verdifull modell for biologisk forskning. Denne protokollen beskriver massespektrometri avbildning av 3D cellekulturer, inkludert forbedringer i prøveopparbeidelse plattformen. Målet med denne protokollen er å instruere brukere å forberede 3D cellekulturer for massespektrometri bildeanalyse.

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