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Bioengineering
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样品制备策略的3D细胞培养模型质谱成像
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
Please note that all translations are automatically generated.
Click here for the English version.
样品制备策略的3D细胞培养模型质谱成像
DOI:
10.3791/52313-v
•
08:14 min
•
December 05, 2014
•
Dorothy R. Ahlf Wheatcraft
2
,
Xin Liu
2
,
Amanda B. Hummon
2
1
Department of Chemistry and Biochemistry
,
University of Notre Dame
,
2
Harper Cancer Research Institute
,
University of Notre Dame
Chapters
00:05
Title
01:28
Three-dimensional Cell Culture and Preparation of Agarose Coated 96 Well Plates
02:32
Seed and Harvest the Three-dimensional Cultures and Embed in Gelatin
04:11
Slice and Thaw Mount the Gelatin-embedded Three-dimensional Cell Cultures for Mass Spectrometric Imaging
05:03
Matrix Application and Sample Preparation for MALDI Imaging
05:42
MALDI-MSI Analysis Using a MALDI-TOF System and Optional Data Analysis Methods
07:28
Results: Mass Spectrometry Imaging of Three-dimensional Cell Cultures
07:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
永生化癌细胞系可以种植三维细胞培养物,生物研究的一个有价值的模型。本协议描述的3D细胞培养,包括改进的样品制备平台质谱成像。该协议的目的是为了指导用户以制备三维细胞培养物用于质谱成像分析。
Tags
3D Cell Culture
Mass Spectrometry Imaging
MALDI-MSI
Sample Preparation
Colon Cancer
Spheroids
Tumor Nodules
Spatial Analysis
Molecular Distribution
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