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February 25, 2015
DOI:
1Department of Biomedical Engineering,Ohio State University Wexner Medical Center, 2Davis Heart & Lung Research Institute,Ohio State University Wexner Medical Center, 3The Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Laboratory,Ohio State University Wexner Medical Center, 4Division of Cardiac Surgery, Department of Surgery,Ohio State University Wexner Medical Center, 5Departments of Pediatrics and Internal Medicine,Ohio State University, 6Advanced Lung Disease Program, Lung and Heart-Lung Transplant Programs,Nationwide Children’s Hospital, 7Division of Transplantation, Department of Surgery,Ohio State University Wexner Medical Center
Summary
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Ex-Vivo Lung Perfusion (EVLP) ha permesso il trapianto di polmone nell'uomo a diventare più facilmente disponibili, consentendo la possibilità di valutare gli organi ed espandere il pool di donatori. Qui, descriviamo lo sviluppo di un programma di topo EVLP e filtri che consentono un modello riproducibile per espansioni future.
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Nelson, K., Bobba, C., Eren, E., Spata, T., Tadres, M., Hayes, Jr., D., Black, S. M., Ghadiali, S., Whitson, B. A. Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program. J. Vis. Exp. (96), e52309, doi:10.3791/52309 (2015).
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