Journal
/
/
真のIgE発現するマウスB系統細胞の検出
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Detection of True IgE-expressing Mouse B Lineage Cells

真のIgE発現するマウスB系統細胞の検出

10,750 Views

09:40 min

December 01, 2014

DOI:

09:40 min
December 01, 2014

11 Views
, , ,

Summary

Automatically generated

In vitro analysis of class switch recombination in mice is challenging due to cytophilic IgE molecules bound to Fc receptors on the surface of B cells. We describe a method for IgE detection using trypsin-mediated cleavage of surface-bound IgE prior to fixation and permeabilization for cytoplasmic fluorescence staining.

Read Article