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Detectie van True IgE-expressie muis B Lineage Cellen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Detection of True IgE-expressing Mouse B Lineage Cells
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Detectie van True IgE-expressie muis B Lineage Cellen

Nederlands

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10,750 Views

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09:40 min

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December 01, 2014

DOI:

10.3791/52264-v

DOI:
10.3791/52264-v

•

09:40 min
December 01, 2014

•

11 Views
, , ,
1Department of Medicine, Division of Rheumatology, Immunology and Allergy,Brigham and Women’s Hospital and Harvard Medical School

Summary

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In vitro analysis of class switch recombination in mice is challenging due to cytophilic IgE molecules bound to Fc receptors on the surface of B cells. We describe a method for IgE detection using trypsin-mediated cleavage of surface-bound IgE prior to fixation and permeabilization for cytoplasmic fluorescence staining.

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Cite this Article

Gallagher, M. P., Shrestha, A., Magee, J. M., Wesemann, D. R. Detection of True IgE-expressing Mouse B Lineage Cells. J. Vis. Exp. (94), e52264, doi:10.3791/52264 (2014).

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