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Método cirúrgico para Virally Mediated Gene entrega à orelha de rato Inner através da janela redonda Membrane
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Neuroscience
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JoVE Journal Neuroscience
Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane

Método cirúrgico para Virally Mediated Gene entrega à orelha de rato Inner através da janela redonda Membrane

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14,833 Views

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07:32 min

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March 16, 2015

DOI:

10.3791/52187-v

DOI:
10.3791/52187-v

•

07:32 min
March 16, 2015

•

16 Views
, , ,
1Department of Otolaryngology-Head and Neck Surgery,University of California, San Francisco

Summary

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The described post-auricular surgical approach allows rapid and direct delivery into the mouse cochlear scala tympani while minimizing blood loss and animal mortality. This method can be used for cochlear therapy using molecular, pharmacologic and viral delivery to postnatal mice through the round window membrane.

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Cite this Article

Akil, O., Rouse, S. L., Chan, D. K., Lustig, L. R. Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane. J. Vis. Exp. (97), e52187, doi:10.3791/52187 (2015).

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