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Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane
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JoVE Journal 神経科学
Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane

Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane

14,822 Views

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07:32 min

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March 16, 2015

DOI:

10.3791/52187-v

DOI:
10.3791/52187-v

•

07:32 min
March 16, 2015

•

16 Views
, , ,
1Department of Otolaryngology-Head and Neck Surgery,University of California, San Francisco

概要

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The described post-auricular surgical approach allows rapid and direct delivery into the mouse cochlear scala tympani while minimizing blood loss and animal mortality. This method can be used for cochlear therapy using molecular, pharmacologic and viral delivery to postnatal mice through the round window membrane.

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Cite this Article

Akil, O., Rouse, S. L., Chan, D. K., Lustig, L. R. Surgical Method for Virally Mediated Gene Delivery to the Mouse Inner Ear through the Round Window Membrane. J. Vis. Exp. (97), e52187, doi:10.3791/52187 (2015).

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