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Hohe Auflösung Elektronenmikroskopie der<em> Helicobacter pylori</em> Cag Typ IV-Sekretionssystem Pili Produziert in variierenden Bedingungen von Iron Verfügbarkeit
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Immunology and Infection
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JoVE Journal Immunology and Infection
High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability
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Hohe Auflösung Elektronenmikroskopie der<em> Helicobacter pylori</em> Cag Typ IV-Sekretionssystem Pili Produziert in variierenden Bedingungen von Iron Verfügbarkeit

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14,760 Views

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09:05 min

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November 21, 2014

DOI:

10.3791/52122-v

DOI:
10.3791/52122-v

•

09:05 min
November 21, 2014

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10 Views
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1Department of Medicine – Division of Infectious Diseases,Vanderbilt University School of Medicine, 2Tennessee Valley Healthcare Systems,U. S. Dept. of Veterans Affairs

Summary

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Here we describe a method to visualize the oncogenic bacterial organelle known as the Cag Type IV Secretion System (Cag-T4SS). We find that the Cag-T4SS is differentially produced on the surface of H. pylori in response to varying conditions of iron availability.

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Haley, K. P., Blanz, E. J., Gaddy, J. A. High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability. J. Vis. Exp. (93), e52122, doi:10.3791/52122 (2014).

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