Live-imaging of the <em>Drosophila</em> Pupal Eye

Mark B. Hellerman; Richard H. Choe; Ruth I. Johnson

doi:10.3791/52120

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Live-imaging of the Drosophila Pupal Eye

JoVE Journal
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Live-imaging of the Drosophila Pupal Eye

Live-imaging of the Drosophila Pupal Eye

9,066 Views

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09:54 min

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January 12, 2015

DOI:

10.3791/52120-v

DOI:

10.3791/52120-v

•

09:54 min

January 12, 2015

•

2 Views

Mark B. Hellerman¹, Richard H. Choe¹, Ruth I. Johnson¹

¹Biology Department,Wesleyan University

Özet

This protocol presents an efficient method for imaging the live Drosophila pupal eye neuroepithelium. This method compensates for tissue movement and uneven topology, enhances visualization of cell boundaries through the use of multiple GFP-tagged junction proteins, and uses an easily-assembled imaging rig.

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Live-imaging of the Drosophila Pupal Eye

Özet

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