/
/
Évaluation de conformation du VIH-1 trimère glycoprotéines d'enveloppe l'aide d'un base de cellules test ELISA
A subscription to JoVE is required to view this content. Sign in or start your free trial.
14,157 Views
•
07:10 min
•
September 14, 2014
DOI:
Summary
Automatically generated
Understanding viral surface antigens conformations is required to evaluate antibody neutralization and guide the design of effective vaccine immunogens. Here we describe a cell-based ELISA assay that allows the study of the recognition of trimeric HIV-1 Env expressed at the surface of transfected cells by specific anti-Env antibodies.
Read Article
Cite this Article
Veillette, M., Coutu, M., Richard, J., Batraville, L., Désormeaux, A., Roger, M., Finzi, A. Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay. J. Vis. Exp. (91), e51995, doi:10.3791/51995 (2014).
Copy