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Immunology and Infection

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JoVE Journal Immunology and Infection
Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
 

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Article DOI: 10.3791/51995-v • 07:10 min • September 14th, 2014
September 14th, 2014 •
1, *1, *1, *1, 1, 1, 1
1Centre de recherche du CHUM, Department of Microbiology, Infectiology and Immunology, Université de Montréal
* These authors contributed equally

Capítulos

Resumo
Automatic Translation

Understanding viral surface antigens conformations is required to evaluate antibody neutralization and guide the design of effective vaccine immunogens. Here we describe a cell-based ELISA assay that allows the study of the recognition of trimeric HIV-1 Env expressed at the surface of transfected cells by specific anti-Env antibodies.

Tags

HIV-1 Envelope Glycoproteins Cell-based ELISA Assay Conformational Evaluation Trimeric Env Monoclonal Antibodies Immunogens Vaccine Strategies Recognition Horseradish Peroxidase-conjugated Secondary Antibody Chemiluminescence Reaction Soluble CD4 Cellular Proteins Medium To High Throughput Screening Antigens
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