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Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
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Biology
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JoVE Journal Biology
Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

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17,099 Views

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08:35 min

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September 11, 2014

DOI:

10.3791/51868-v

DOI:
10.3791/51868-v

•

08:35 min
September 11, 2014

•

2 Views
, , ,
1Fachbereich Biologie, Entwicklungsbiologie,Philipps-Universität Marburg, 2Institut für Molekularbiologie und Tumorforschung,Philipps-Universität Marburg

Summary

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This protocol describes the dissection and cultivation of intact testes and germ-line cysts from Drosophila melanogaster pupae. This method allows microscopic observation of spermatogenesis ex vivo. Furthermore, we describe a pharmacological assay of the effect of inhibitors on specific stages of germ-cell development in pupal testes.

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Cite this Article

Gärtner, S. M. K., Rathke, C., Renkawitz-Pohl, R., Awe, S. Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment. J. Vis. Exp. (91), e51868, doi:10.3791/51868 (2014).

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