ジャーナル
/
生物学
/
Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

17,117 Views

•

08:35 min

•

September 11, 2014

DOI:

10.3791/51868-v

DOI:
10.3791/51868-v

•

08:35 min
September 11, 2014

•

2 Views
, , ,
1Fachbereich Biologie, Entwicklungsbiologie,Philipps-Universität Marburg, 2Institut für Molekularbiologie und Tumorforschung,Philipps-Universität Marburg

概要

Automatically generated

This protocol describes the dissection and cultivation of intact testes and germ-line cysts from Drosophila melanogaster pupae. This method allows microscopic observation of spermatogenesis ex vivo. Furthermore, we describe a pharmacological assay of the effect of inhibitors on specific stages of germ-cell development in pupal testes.

Explore More Videos

DrosophilaPupal TestisEx Vivo CultureGerm-line CystsSpermatogenesisPost-meioticHistone-to-protamine SwitchPharmacological TreatmentMicroscopic Imaging

Read Article

Cite this Article

Gärtner, S. M. K., Rathke, C., Renkawitz-Pohl, R., Awe, S. Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment. J. Vis. Exp. (91), e51868, doi:10.3791/51868 (2014).

Download .ris file

Copy

Share Video

.

Copy