The ability to monitor T cell responses in detail in vivo is important for the development of our understanding of the immune response. Here we describe the use of fluorescent target arrays (FTAs) in an in vivo T cell assay that assesses >250 parameters simultaneously by flow cytometry.
Quah, B. J. C., Wijesundara, D. K., Ranasinghe, C., Parish, C. R. The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo. J. Vis. Exp. (88), e51627, doi:10.3791/51627 (2014).