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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
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Biology
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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

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23,866 Views

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06:36 min

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July 17, 2014

DOI:

10.3791/51604-v

DOI:
10.3791/51604-v

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06:36 min
July 17, 2014

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4 Views
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1Department of Biological Sciences,University of Notre Dame

Summary

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The zebrafish embryo is an excellent model for developmental biology research. During embryogenesis, zebrafish develop with a yolk mass, which presents three-dimensional challenges for sample observation and analysis. This protocol describes how to create two-dimensional flat mount preparations of whole mount in situ (WISH) stained zebrafish embryo specimens.

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Cite this Article

Cheng, C. N., Li, Y., Marra, A. N., Verdun, V., Wingert, R. A. Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization. J. Vis. Exp. (89), e51604, doi:10.3791/51604 (2014).

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