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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
JoVE Journal
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JoVE Journal Biyoloji
Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

DOI:
10.3791/51604-v

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06:36 min

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July 17, 2014

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, , , ,
1Department of Biological Sciences,Notre Dame Üniversitesi

Bölümler

  • 00:05Başlık
  • 01:37Dissection and Initial Deyolking of Embryo
  • 02:26Fine Deyolking of the Embryo
  • 03:18Mounting Embryo on a Glass Slide
  • 04:23Results: In situ Hybridization of Flat-mounted Embryos
  • 05:58Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The zebrafish embryo is an excellent model for developmental biology research. During embryogenesis, zebrafish develop with a yolk mass, which presents three-dimensional challenges for sample observation and analysis. This protocol describes how to create two-dimensional flat mount preparations of whole mount in situ (WISH) stained zebrafish embryo specimens.

Etiketler

Zebrafish EmbryoDevelopmental StagesWhole Mount In Situ Hybridization (WISH)Flat Mount PreparationGene ExpressionEmbryonic StructuresGelişim BiyolojisiBiomedical Research

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