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July 01, 2014
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Summary
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Stable isotope labeling of peptides by reductive dimethylation (ReDi labeling) is a rapid, inexpensive strategy for accurate mass spectrometry-based quantitative proteomics. Here we demonstrate a robust method for preparation and analysis of protein mixtures using the ReDi approach that can be applied to nearly any sample type.
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Tolonen, A. C., Haas, W. Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling. J. Vis. Exp. (89), e51416, doi:10.3791/51416 (2014).
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