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Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
章
- 00:05標題
- 01:19Protein Purification and Alkylation of Free Sulfhydryl Groups
- 02:59Protein Digestion, Reversed Phase Peptide Extraction, and Reductive Dimethylation Labeling
- 06:02Basic pH Reversed Phase Chromatography and STop and Go Extraction
- 08:17Microcapillary LC-MS/MS and Peptide Quantification
- 09:58Results: Accuracy, Precision, and Reproducibility of ReDi Labeling
- 11:24Conclusion
Stable isotope labeling of peptides by reductive dimethylation (ReDi labeling) is a rapid, inexpensive strategy for accurate mass spectrometry-based quantitative proteomics. Here we demonstrate a robust method for preparation and analysis of protein mixtures using the ReDi approach that can be applied to nearly any sample type.
