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在Wholemount大鼠视网膜免疫组化和钙成像方法
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Immunohistochemical and Calcium Imaging Methods in Wholemount Rat Retina
DOI:

08:54 min

October 13, 2014

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Chapters

  • 00:05Title
  • 01:25Eyecup Preparation
  • 02:58Wholemount Retina Preparation and Processing
  • 05:06Calcium Imaging in Retinal Ganglion Cells
  • 07:19Results: RGC Immunostaining and Electrophysiology
  • 08:29Conclusion

Summary

Automatic Translation

免疫组化协议,用于研究在视网膜特定蛋白质的定位。钙成像技术被用来研究视网膜神经节细胞及其轴突钙的动态。

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