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一个流程粘附实验来研究白细胞招聘剪切应力的人力肝窦内皮细胞条件下
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress
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一个流程粘附实验来研究白细胞招聘剪切应力的人力肝窦内皮细胞条件下

DOI:
10.3791/51330-v

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11:38 min

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March 21, 2014

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, , ,
1NIHR Biomedical Research Unit, Centre for Liver Research, School of Immunity and Infection,University of Birmingham

Chapters

  • 00:05Title
  • 01:59Cytokine Stimulation of Endothelial Cells
  • 04:11Isolation and Pretreatment of Peripheral Blood Lymphocytes
  • 06:09Setup of Flow Assay System
  • 08:33Flow AssayTechnique and Recording of Adhesion for Analysis
  • 09:35Results: Adhesion Cascade of Blood Lymphocytes over Hepatic Sinusoidal Endothelium
  • 10:44Conclusion

Summary

Automatic Translation

Automatic Translation

白细胞募集到肝脏发生这是由独特的肝窦内皮细胞衬里肝窦的专业渠道内。整个生理切应力条件下的人力肝窦内皮细胞白细胞招聘相衬显微镜可以促进其背后这一过程的分子机制的阐明。

Tags

Keywords: Flow Adhesion AssayLeucocyte RecruitmentHuman Hepatic Sinusoidal EndotheliumShear StressInflammatory Liver DiseasesFibrosisCirrhosisMolecular MechanismsTherapeutic TargetsLeucocyte InfiltrationHepatic SinusoidsHepatic Sinusoidal Endothelial Cells (HSEC)Adhesion CascadeTetheringActivationFirm AdhesionCrawlingTransmigrationPhase Contrast MicroscopyInhibitors

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