Journal
/
Immunologie und Infektion
/
Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal Immunologie und Infektion
Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets

Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets

36,654 Views

•

11:40 min

•

March 27, 2014

DOI:

10.3791/51301-v

DOI:
10.3791/51301-v

•

11:40 min
March 27, 2014

•

7 Views
, , , , ,
1UAB Mitochondrial Medicine Laboratory, Center for Free Radical Biology, Department of Pathology,University of Alabama at Birmingham

Summary

Automatically generated

Blood leukocytes and platelets can be used as a marker of overall bioenergetic health of an individual and so have the potential to monitor pathological processes and the impact of treatments. Here we describe a method to isolate and measure mitochondrial function and the oxidative burst in these cells.

Explore More Videos

BioenergeticsOxidative BurstLeukocytesPlateletsMitochondrial DysfunctionAtherosclerosisDiabetesSeptic ShockNeurodegenerative DiseasesHyperglycemiaInflammationMagnetic Bead IsolationCell AdhesionMicroplate RespirometryExtracellular Flux AnalyzerMonocytesLymphocytesNeutrophilsReactive Oxygen Species

Read Article

Cite this Article

Kramer, P. A., Chacko, B. K., Ravi, S., Johnson, M. S., Mitchell, T., Darley-Usmar, V. M. Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets. J. Vis. Exp. (85), e51301, doi:10.3791/51301 (2014).

Download .ris file

Copy

Share Video

.

Copy