Journal
/
Immunology and Infection
/
Activering en waardering van NLRP3 inflammasome activiteit met behulp van IL-1β in Human Monocyt-afgeleide dendritische cellen
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
Please note that all translations are automatically generated. Click here for the English version.

Activering en waardering van NLRP3 inflammasome activiteit met behulp van IL-1β in Human Monocyt-afgeleide dendritische cellen

Nederlands

Automatically Generated

20,633 Views

•

09:04 min

•

May 22, 2014

DOI:

10.3791/51284-v

DOI:
10.3791/51284-v

•

09:04 min
May 22, 2014

•

4 Views
, ,
1Department of Pathology,New York University School of Medicine, 2Division of Infectious Diseases, Department of Medicine,Mount Sinai Medical Center, 3Division of Hematology and Oncology, Hess Center for Science and Medicine,Mount Sinai Medical Center

Summary

Automatically generated

Dendritische cellen (DCs) scheiden IL-1β reactie op TLR8 erkenning van synthetische purine, R848, gevolgd door NLRP3 inflammasome activering met nigericine derhalve IL-1β kunnen worden gebruikt om NLRP3 inflammasome te meten. Intracellulaire cytokine kleuring, immunoblotting en ELISA worden gebruikt om NLRP3 inflammasome priming en activering van IL-1β expressie nauwkeurig te meten.

Explore More Videos

NLRP3 InflammasomeIL-1βMonocyte-derived Dendritic CellsR848NigericinInnate Immune ResponseInflammationCaspase-1TLR8Bacterial ToxinPotassium Ionophore

Read Article

Cite this Article

Fernandez, M. V., Miller, E. A., Bhardwaj, N. Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells. J. Vis. Exp. (87), e51284, doi:10.3791/51284 (2014).

Download .ris file

Copy

Share Video

.

Copy