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抗核抗体筛选使用Hep-2细胞
JoVE Journal
Biology
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JoVE Journal Biology
Anti-Nuclear Antibody Screening Using HEp-2 Cells
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抗核抗体筛选使用Hep-2细胞

English

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132,438 Views

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13:01 min

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June 23, 2014

DOI:

10.3791/51211-v

DOI:
10.3791/51211-v

•

13:01 min
June 23, 2014

•

39 Views
, , ,
1IFA Development Manager,INOVA Diagnostics, Inc., 2IFA Development,INOVA Diagnostics, Inc., 3Product Manager, Rheumatology,INOVA Diagnostics, Inc., 4Medical Director,INOVA Diagnostics, Inc.

Summary

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间接免疫荧光（IIF）的测定法在传统上被用于抗核抗体（ANA）的人血清中的检测。这些抗体的存在可以在全身性自身免疫性风湿病（SARD）帮助诊断。该协议演示了如何有效地进行IIF技术，准确检测这些自身抗体。

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Cite this Article

Buchner, C., Bryant, C., Eslami, A., Lakos, G. Anti-Nuclear Antibody Screening Using HEp-2 Cells. J. Vis. Exp. (88), e51211, doi:10.3791/51211 (2014).

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