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Hematopoetik progenitörlerin Evlatlık Transferi kullanarak Peter Parçacıklannda Murin plazmasitoid dendritik hücreleri Gelişimini değerlendirilmesi
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors
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Hematopoetik progenitörlerin Evlatlık Transferi kullanarak Peter Parçacıklannda Murin plazmasitoid dendritik hücreleri Gelişimini değerlendirilmesi

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11,074 Views

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13:34 min

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March 17, 2014

DOI:

10.3791/51189-v

DOI:
10.3791/51189-v

•

13:34 min
March 17, 2014

•

3 Views
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1Department of Immunology,The University of Texas MD Anderson Cancer Center, 2The University of Texas Graduate School of Biomedical Sciences

Summary

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Bu protokol, FACS-aracılı hücre izolasyon hidrodinamik gen transferi, ve Peyer yama bağışıklık alt-akışı analizi içeren teknikleri kullanarak, ortak bir dendritik hücre progenitörlerinden Peyer yama plazmasitoid dendritik hücrelerin farklılaşmasını değerlendirmek için deneysel prosedürler açıklanmaktadır.

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Cite this Article

Li, H. S., Watowich, S. S. Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors. J. Vis. Exp. (85), e51189, doi:10.3791/51189 (2014).

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