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Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors
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Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors
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March 17, 2014
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This protocol describes experimental procedures to assess the differentiation of plasmacytoid dendritic cells in Peyer’s patch from common dendritic cell progenitors, using techniques involving FACS-mediated cell isolation, hydrodynamic gene transfer, and flow analysis of immune subsets in Peyer’s patch.
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Li, H. S., Watowich, S. S. Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors. J. Vis. Exp. (85), e51189, doi:10.3791/51189 (2014).
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