This content is Free Access.
Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
10,433 Views
•
11:46 min
•
January 13, 2014
DOI:
概要
Automatically generated
High-quality total RNA has been prepared from cell bodies of mouse spinal cord motor neurons by laser capture microdissection after staining spinal cord sections with Azure B in 70% ethanol. Sufficient RNA (~40-60 ng) is recovered from 3,000-4,000 motor neurons to allow downstream RNA analysis by RNA-seq and qRT-PCR.
Read Article
Cite this Article
Bandyopadhyay, U., Fenton, W. A., Horwich, A. L., Nagy, M. Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection. J. Vis. Exp. (83), e51168, doi:10.3791/51168 (2014).
Copy