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Usando proteínas fluorescentes para visualizar e Quantificar<em> Chlamydia</em> Vacúolo Crescimento Dynamics em células vivas
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Immunology and Infection
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JoVE Journal Immunology and Infection
Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells

Usando proteínas fluorescentes para visualizar e Quantificar<em> Chlamydia</em> Vacúolo Crescimento Dynamics em células vivas

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7,414 Views

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07:42 min

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October 13, 2015

DOI:

10.3791/51131-v

DOI:
10.3791/51131-v

•

07:42 min
October 13, 2015

•

2 Views
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1Division of Allergy and Infectious Diseases, Department of Medicine,University of Washington, 2Program in Infectious Diseases, School of Public Health,University of California at Berkeley

Summary

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A live cell fluorescent protein based method for illuminating cellular vacuoles (inclusions) containing Chlamydia is described. This strategy enables rapid, automated determination of Chlamydia infectivity in samples and can be used to quantitatively investigate inclusion growth dynamics.

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Cite this Article

Zuck, M., Feng, C., Hybiske, K. Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells. J. Vis. Exp. (104), e51131, doi:10.3791/51131 (2015).

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