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連続の設計および動作<sup> 13</sup> Cと<sup> 15</sup均一または差動、代謝および構造·> N標識化会議所、工場同位体ラベル化
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Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
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連続の設計および動作<sup> 13</sup> Cと<sup> 15</sup均一または差動、代謝および構造·> N標識化会議所、工場同位体ラベル化

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21,296 Views

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10:16 min

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January 16, 2014

DOI:

10.3791/51117-v

DOI:
10.3791/51117-v

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10:16 min
January 16, 2014

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7 Views
, , , , , ,
1Natural Resource Ecology Laboratory,Colorado State University, 2Soil Plant Nutrient REsearch,USDA-ARS, 3Department of Soil and Crop Sciences,Colorado State University

Summary

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この方法は、連続13℃で均一または差動植物組織標識用15 N同位体標識室を構築し、操作する方法について説明します。代謝およびAndropogonのgerardiiの構造的な標識からの代表的な結果が説明されています。

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Cite this Article

Soong, J. L., Reuss, D., Pinney, C., Boyack, T., Haddix, M. L., Stewart, C. E., Cotrufo, M. F. Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling. J. Vis. Exp. (83), e51117, doi:10.3791/51117 (2014).

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