Design and Operation of a Continuous <sup>13</sup>C and <sup>15</sup>N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

Jennifer L Soong; Dan Reuss; Colin Pinney; Ty Boyack; Michelle L Haddix; Catherine E Stewart; M. Francesca Cotrufo

doi:10.3791/51117

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Design and Operation of a Continuous ¹³C and ¹⁵N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

JoVE Journal
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JoVE Journal Ambiente

Design and Operation of a Continuous ¹³C and ¹⁵N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

21,296 Views

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10:16 min

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January 16, 2014

DOI:

10.3791/51117-v

DOI:

10.3791/51117-v

•

10:16 min

January 16, 2014

•

7 Views

Jennifer L Soong¹, Dan Reuss¹, Colin Pinney¹, Ty Boyack¹, Michelle L Haddix¹, Catherine E Stewart², M. Francesca Cotrufo^1,3

¹Natural Resource Ecology Laboratory,Colorado State University, ²Soil Plant Nutrient REsearch,USDA-ARS, ³Department of Soil and Crop Sciences,Colorado State University

Summary

This method explains how to build and operate a continuous ¹³C and ¹⁵N isotope labeling chamber for uniform or differential plant tissue labeling. Representative results from metabolic and structural labeling of Andropogon gerardii are discussed.

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Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

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Design and Operation of a Continuous ¹³C and ¹⁵N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling