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Visualização do imunológica Synapse por Dual Color Time-fechado Emissão Estimulada Exaustão (STED) Nanoscopia
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

Visualização do imunológica Synapse por Dual Color Time-fechado Emissão Estimulada Exaustão (STED) Nanoscopia

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77,810 Views

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10:00 min

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March 24, 2014

DOI:

10.3791/51100-v

DOI:
10.3791/51100-v

•

10:00 min
March 24, 2014

•

1 Views
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1Center for Human Immunobiology,Texas Children’s Hospital and Baylor College of Medicine

Summary

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Aqui, ilustramos o protocolo para a imagiologia por duas cores STED Nanoscopia citotóxica sinapse imune das células NK retomados em vidro. Usando este método, obtemos resolução nm sub-100 de proteínas de sinapses e do citoesqueleto.

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Cite this Article

Mace, E. M., Orange, J. S. Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy. J. Vis. Exp. (85), e51100, doi:10.3791/51100 (2014).

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