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Visualisierung der immunologischen Synapse von Dual Color Zeitgesteuerte Stimulated Emission Depletion (STED) Nanoskopie
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
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Visualisierung der immunologischen Synapse von Dual Color Zeitgesteuerte Stimulated Emission Depletion (STED) Nanoskopie

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77,810 Views

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10:00 min

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March 24, 2014

DOI:

10.3791/51100-v

DOI:
10.3791/51100-v

•

10:00 min
March 24, 2014

•

1 Views
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1Center for Human Immunobiology,Texas Children’s Hospital and Baylor College of Medicine

Summary

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Hier veranschaulichen wir das Protokoll für die Bildgebung durch Zwei-Farben-STED Nanoskopie die zytotoxische Immunsynapse von NK-Zellen auf Glas rekapituliert. Mit dieser Methode erhalten wir sub-100 nm Auflösung von Synapsen-Proteine ​​und des Zytoskeletts.

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Cite this Article

Mace, E. M., Orange, J. S. Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy. J. Vis. Exp. (85), e51100, doi:10.3791/51100 (2014).

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