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JoVE Journal Neuroscience
Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
 

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Article DOI: 10.3791/51070-v • 09:07 min • March 17th, 2014
March 17th, 2014 •
1,2, 1,2, 1,2, 1,2
1Cellular and Molelcular Neurobiology, Max Planck Institute of Experimental Medicine, 2Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)

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Neuronal morphogenesis and migration are crucial events underlying proper brain development. Here, we describe methods to genetically manipulate cultured cerebellar granule neurons and the developing cerebellum for the assessment of morphology and migratory characteristics of neurons.

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Genetic Manipulation Cerebellar Granule Neurons In Vitro In Vivo Neuronal Morphology Migration Developmental Events RNA Interference Overexpression Small Molecules Axonogenesis Dendritogenesis Rodent Cerebellar Cortex Electroporation Technique
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