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整个老鼠大脑与光共聚焦显微镜板微米级分辨率光学层析成像

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

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整个老鼠大脑与光共聚焦显微镜板微米级分辨率光学层析成像

16,355 Views

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09:49 min

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October 08, 2013

DOI:

10.3791/50696-v

DOI:

10.3791/50696-v

•

09:49 min

October 08, 2013

•

5 Views

Ludovico Silvestri¹, Alessandro Bria^2,3, Irene Costantini¹, Leonardo Sacconi^1,4, Hanchuan Peng⁵, Giulio Iannello², Francesco Saverio Pavone^1,4,6,7

¹European Laboratory for Non-linear Spectroscopy (LENS), ²Integrated Research Centre,University Campus Bio-medico of Rome, ³DAEMI,University of Cassino, ⁴National Institute of Optics (CNR-INO), ⁵Allen Institute for Brain Science, ⁶Department of Physics,University of Florence, ⁷ICON Foundation, Sesto Fiorentino, Italy

Summary

在这篇文章中，我们描述了完整的实验程序来重建，具有分辨率高，荧光标记的小鼠大脑的优良大脑解剖。所描述的协议包括样品制备和清算，样品安装成像，数据后处理和多尺度可视化。

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