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Neuroscience

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JoVE Journal Neuroscience
Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
 

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Article DOI: 10.3791/50696-v • 09:49 min • October 8th, 2013
October 8th, 2013 •
1, 2,3, 1, 1,4, 5, 2, 1,4,6,7
1European Laboratory for Non-linear Spectroscopy (LENS), 2Integrated Research Centre, University Campus Bio-medico of Rome, 3DAEMI, University of Cassino, 4National Institute of Optics (CNR-INO), 5Allen Institute for Brain Science, 6Department of Physics, University of Florence, 7ICON Foundation, Sesto Fiorentino, Italy

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In this article we describe the full experimental procedure to reconstruct, with high resolution, the fine brain anatomy of fluorescently labeled mouse brains. The described protocol includes sample preparation and clearing, specimen mounting for imaging, data post-processing and multi-scale visualization.

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Micron-scale Resolution Optical Tomography Entire Mouse Brains Confocal Light Sheet Microscopy Neuroscience Neuronal Soma Projections Imaging Technologies Data Management Macroscopic Volumes High Resolution High Contrast TeraByte Range Datasets Confocal Light Sheet Microscopy (CLSM) Enhanced Green Fluorescent Protein (EGFP) Light Sheet Illumination Confocal Detection Deep Imaging Cleared Specimens Experimental Pipeline Fluorescent Proteins Clearing Procedures Mounting Procedures Optical Tomography Parallel Adjacent Stacks Open-source Software Tools Stitching Multiple Stacks Multi-resolution Data Navigation
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