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Komparativen Ansatz, um die Landschaft von Wirt-Pathogen-Protein-Protein-Wechselwirkungen charakterisieren
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
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Komparativen Ansatz, um die Landschaft von Wirt-Pathogen-Protein-Protein-Wechselwirkungen charakterisieren

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11,056 Views

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13:56 min

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July 18, 2013

DOI:

10.3791/50404-v

DOI:
10.3791/50404-v

•

13:56 min
July 18, 2013

•

2 Views
, , , ,
1Unité de Génétique, Papillomavirus et Cancer Humain (GPCH),Institut Pasteur , 2Cellule Pasteur,Université Sorbonne Paris Cité, 3Center for Cancer Systems Biology (CCSB), Harvard Medical School, Department of Cancer Biology,Dana Farber Cancer Institute

Summary

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Dieser Artikel konzentriert sich auf die Identifizierung von High-Interaktion zuversichtlich Datensätze zwischen Wirt und Pathogen-Proteine ​​mit einer Kombination aus zwei orthogonal Methoden: Hefe-Zwei-Hybrid von einem High-Throughput-Assay Interaktion in Säugerzellen namens HT-GPCA gefolgt.

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Cite this Article

Muller, M., Cassonnet, P., Favre, M., Jacob, Y., Demeret, C. A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions. J. Vis. Exp. (77), e50404, doi:10.3791/50404 (2013).

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